The country star and international treasure posted pictures of her being taken on Friday on Twitter.
She thanked the staff at Vanderbilt University Medical Center and wrote: “Dolly receives a (second) dose of her own medicine”.
A small blue bandage was showing from the shoulder cutout on his sweater in one of the photos.
Parton, 75, donated $ 1 million to the Covid-19 research efforts at the Nashville, Tennessee hospital last year, which helped finance the development of the Modern vaccine.
She received her first dose of the vaccine on March 2 and posted a video on Twitter asking everyone to get the vaccines when they can, “because the sooner we start feeling better, the sooner we will be back to normal.”
Parton even reworked the lyrics to his hit song “Jolene” for the occasion.
“Vaccine, vaccine, vaccine, vaccine”, she sings in the video. “I’m begging you, please don’t hesitate.”
On Monday, Tennessee will open eligibility for the vaccine to all residents of the state over the age of 16.
Tennessee administered more than 2.8 million doses of the vaccine and about 23% of the state’s population received at least one dose, according to the state health department.